Dear Tobias, I have 97 samples to analyze with SEQCAP, but it always failed when it just finished 81 samples. The error is weird: ```python multiprocessing.pool.RemoteTraceback: """ Traceback (most recent call last): File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/multiprocessing/pool.py", line 125, in worker result = (True, func(*args, **kwds)) File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/multiprocessing/pool.py", line 48, in mapstar return list(map(*args)) File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/site-packages/secapr/assemble_reads.py", line 288, in process_subfolder os.makedirs(out_dir) File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/os.py", line 223, in makedirs mkdir(name, mode) FileExistsError: [Errno 17] File exists: '/home/gmeng/work/4.assembly/secapr/April-5-2022/stats' """ The above exception was the direct cause of the following exception: Traceback (most recent call last): File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/bin/secapr", line 10, in <module> sys.exit(main()) File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/site-packages/secapr/__main__.py", line 55, in main args.func(args) File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/site-packages/secapr/assemble_reads.py", line 381, in main contig_count_df_list = list(pool.map(partial(process_subfolder), pool_args)) File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/multiprocessing/pool.py", line 364, in map return self._map_async(func, iterable, mapstar, chunksize).get() File "/home/gmeng/.conda/envs/mybase/envs/secapr_env/lib/python3.8/multiprocessing/pool.py", line 771, in get raise self._value FileExistsError: [Errno 17] File exists: '/home/gmeng/work/4.assembly/secapr/April-5-2022/stats' ``` because the `/home/gmeng/work/4.assembly/secapr/April-5-2022/stats` path should have been created at the very beginning. It seems that it is similar to https://github.com/Theano/Theano/issues/6610. Currently, I can't figure out the exact reasons for this error, so I just simply rerun the unfinished samples as I talked about in https://github.com/AntonelliLab/seqcap_processor/issues/26. Cheers Guanliang
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